Hotspot

psbM-tRNA-Tyr(GUA)

LSC · 32608-34300 · 1693 bp

Back to Najas

Pi 0.0795
MarkerSeek score 87.00
Primer available yes
Label psbM-tRNA-Tyr(GUA)

Feature Metadata

label_name
psbM-tRNA-Tyr(GUA)
feature_type
igs
region
LSC
length_bp
1693
variable_sites
125
pi
0.0795
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
87.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-tRNA-Tyr(GUA)_p1 AGTAAAAAGGCAGTCGGAACT GGGGACGGACTGTAAATTCG 42.9 55.0 57.5 58.4 1797-1822 bp 1.0 74.8
psbM-tRNA-Tyr(GUA)_p2 AGTAAAAAGGCAGTCGGAACT GGGACGGACTGTAAATTCGT 42.9 50.0 57.5 57.6 1796-1821 bp 1.0 74.6
psbM-tRNA-Tyr(GUA)_p3 AAAAGGCAGTCGGAACTAGA GGGGACGGACTGTAAATTCG 45.0 55.0 56.5 58.4 1793-1818 bp 1.0 74.6
psbM-tRNA-Tyr(GUA)_p4 AAAGGCAGTCGGAACTAGAA GGGGACGGACTGTAAATTCG 45.0 55.0 56.5 58.4 1792-1817 bp 1.0 74.6
psbM-tRNA-Tyr(GUA)_p5 AAAGGCAGTCGGAACTAGAA GGGACGGACTGTAAATTCGT 45.0 50.0 56.5 57.6 1791-1816 bp 1.0 74.4