Hotspot

petN-psbM

LSC · 31805-32502 · 698 bp

Back to Najas

Pi 0.0639
MarkerSeek score 87.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
698
variable_sites
41
pi
0.0639
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
87.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTATGGGGAAGGAGTGGAC AGTTCCGACTGCCTTTTTACT 55.0 42.9 58.1 57.5 747-781 bp 1.0 75.4
petN-psbM_p2 GTATGGGGAAGGAGTGGACT AGTTCCGACTGCCTTTTTACT 55.0 42.9 58.1 57.5 746-780 bp 1.0 75.4
petN-psbM_p3 TATGGGGAAGGAGTGGACTG AGTTCCGACTGCCTTTTTACT 55.0 42.9 58.1 57.5 745-779 bp 1.0 75.4
petN-psbM_p4 GTATGGGGAAGGAGTGGACT TCTAGTTCCGACTGCCTTTT 55.0 45.0 58.1 56.5 749-783 bp 1.0 75.2
petN-psbM_p5 GTATGGGGAAGGAGTGGACT TTCTAGTTCCGACTGCCTTT 55.0 45.0 58.1 56.5 750-784 bp 1.0 75.2