Hotspot

tRNA-Ser(GCU)-tRNA-Gly(UCC)

LSC · 10126-11243 · 1118 bp

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Pi 0.0833
MarkerSeek score 86.30
Primer available yes
Label tRNA-Ser(GCU)-tRNA-Gly(UCC)

Feature Metadata

label_name
tRNA-Ser(GCU)-tRNA-Gly(UCC)
feature_type
igs
region
LSC
length_bp
1118
variable_sites
88
pi
0.0833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1187-1192 bp 1.0 61.6
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1186-1191 bp 1.0 61.6
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 1190-1195 bp 1.0 60.5
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1189-1194 bp 1.0 60.5
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1188-1193 bp 1.0 60.5