Hotspot

rps16-tRNA-Gln(UUG)

LSC · 6681-8301 · 1621 bp

Back to Najas

Pi 0.121
MarkerSeek score 85.50
Primer available yes
Label rps16-tRNA-Gln(UUG)

Feature Metadata

label_name
rps16-tRNA-Gln(UUG)
feature_type
igs
region
LSC
length_bp
1621
variable_sites
171
pi
0.121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-tRNA-Gln(UUG)_p1 GTGTCCTTCAAGTCACACGT AAGGTTCGAATCCTTCCGTC 50.0 50.0 58.1 57.6 1592-1703 bp 1.0 88.4
rps16-tRNA-Gln(UUG)_p2 GTGTCCTTCAAGTCACACGT TAAATGGAAATGGGGCGTGG 50.0 50.0 58.1 58.5 1650-1761 bp 1.0 87.3
rps16-tRNA-Gln(UUG)_p3 ACACGTTGCTTTCTACCACA AAGGTTCGAATCCTTCCGTC 45.0 50.0 57.7 57.6 1578-1689 bp 1.0 86.4
rps16-tRNA-Gln(UUG)_p4 GTGTCCTTCAAGTCACACGT TAAGGCAACGGGTTTTGGTC 50.0 50.0 58.1 58.7 1622-1733 bp 1.0 86.1
rps16-tRNA-Gln(UUG)_p5 ACACGTTGCTTTCTACCACA TAAATGGAAATGGGGCGTGG 45.0 50.0 57.7 58.5 1636-1747 bp 1.0 85.3