Hotspot

tRNA-Lys(UUU)-rps16

LSC · 4253-5003 · 751 bp

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Pi 0.12
MarkerSeek score 82.30
Primer available yes
Label tRNA-Lys(UUU)-rps16

Feature Metadata

label_name
tRNA-Lys(UUU)-rps16
feature_type
igs
region
LSC
length_bp
751
variable_sites
81
pi
0.12
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys(UUU)-rps16_p1 TCCCTGGAATTGGAGCCTAA TCTTGAAAAGGGGGCTCAAC 50.0 50.0 58.0 58.0 1087-1124 bp 1.0 90.8
tRNA-Lys(UUU)-rps16_p2 TTCCCTGGAATTGGAGCCTA TCTTGAAAAGGGGGCTCAAC 50.0 50.0 58.0 58.0 1088-1125 bp 1.0 90.8
tRNA-Lys(UUU)-rps16_p3 TGAAGAACACAAGCCCCTTC TCTTGAAAAGGGGGCTCAAC 50.0 50.0 58.0 58.0 1116-1153 bp 1.0 90.7
tRNA-Lys(UUU)-rps16_p4 TGTTTCCCTGGAATTGGAGC TCTTGAAAAGGGGGCTCAAC 50.0 50.0 58.1 58.0 1091-1128 bp 1.0 90.3
tRNA-Lys(UUU)-rps16_p5 CCCCAAGAATCAAAGCGAGA TCTTGAAAAGGGGGCTCAAC 50.0 50.0 57.9 58.0 1052 bp 0.5 59.1