Hotspot

ycf2-trnI-CAU

Genome · 129365-129911 · 547 bp

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Pi 0.0293
MarkerSeek score 60.30
Primer available yes
Label ycf2-trnI-CAU

Feature Metadata

label_name
ycf2-trnI-CAU
feature_type
igs
region
Genome
length_bp
547
variable_sites
16
pi
0.0293
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnI-CAU_p1 CGGTTCTTGTCTCGGTTCAT TACCATTCAGCCATGGATGC 50.0 50.0 57.9 58.0 587-687 bp 1.0 81.4
ycf2-trnI-CAU_p2 CGGTTCTTGTCTCGGTTCAT CCGCGACTTCCCAATTATGA 50.0 50.0 57.9 58.1 619-719 bp 1.0 81.0
ycf2-trnI-CAU_p3 CGGTTCTTGTCTCGGTTCAT CCACAGGCTATTGGAAGACC 50.0 55.0 57.9 58.0 660-760 bp 1.0 80.9
ycf2-trnI-CAU_p4 CGGTTCTTGTCTCGGTTCAT ACGACCCGACTTAGCTATCA 50.0 50.0 57.9 58.0 1148-1248 bp 1.0 80.5
ycf2-trnI-CAU_p5 CGGTTCTTGTCTCGGTTCAT GAATGGCTCATGCTAACCCA 50.0 50.0 57.9 57.9 1125-1225 bp 1.0 80.5