Hotspot

ndhB-trnL-CAA

Genome · 122013-122281 · 269 bp

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Pi 0.0192
MarkerSeek score 66.70
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
Genome
length_bp
269
variable_sites
5
pi
0.0192
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 GCAATTGCTCAGGAGACCTT GTCTCGCGTGTCTACCATTT 50.0 50.0 58.2 58.0 319-328 bp 1.0 82.1
ndhB-trnL-CAA_p2 GCAATTGCTCAGGAGACCTT CTCGCGTGTCTACCATTTCA 50.0 50.0 58.2 58.0 317-326 bp 1.0 82.1
ndhB-trnL-CAA_p3 GCAATTGCTCAGGAGACCTT GCGTGTCTACCATTTCACCA 50.0 50.0 58.2 58.2 314-323 bp 1.0 80.6
ndhB-trnL-CAA_p4 GCAATTGCTCAGGAGACCTT TCTCGCGTGTCTACCATTTC 50.0 50.0 58.2 57.7 318-327 bp 1.0 79.9
ndhB-trnL-CAA_p5 GCAATTGCTCAGGAGACCTT TCGCGTGTCTACCATTTCAC 50.0 50.0 58.2 58.3 316-325 bp 1.0 79.8