Hotspot

rrn16-trnV-GAC

Genome · 115304-116752 · 1449 bp

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Pi 0.0174
MarkerSeek score 60.40
Primer available yes
Label rrn16-trnV-GAC

Feature Metadata

label_name
rrn16-trnV-GAC
feature_type
igs
region
Genome
length_bp
1449
variable_sites
25
pi
0.0174
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnV-GAC_p1 ATGTGTTAAGCATGCCACCA GACGTGGTGATAGTCATCGG 45.0 55.0 58.1 57.9 1536-1539 bp 1.0 80.2
rrn16-trnV-GAC_p2 CCGACTTGCATGTGTTAAGC GACGTGGTGATAGTCATCGG 50.0 55.0 57.8 57.9 1545-1548 bp 1.0 78.9
rrn16-trnV-GAC_p3 CATGTGTTAAGCATGCCACC GACGTGGTGATAGTCATCGG 50.0 55.0 57.7 57.9 1537-1540 bp 1.0 78.6
rrn16-trnV-GAC_p4 GCAGGTTCTTACGCGTTACT GACGTGGTGATAGTCATCGG 50.0 55.0 58.3 57.9 1598-1601 bp 1.0 78.4
rrn16-trnV-GAC_p5 TTCTTACGCGTTACTCACCC GACGTGGTGATAGTCATCGG 50.0 55.0 57.6 57.9 1593-1596 bp 1.0 78.0