Hotspot

trnI-GAU-rrn16

Genome · 113189-113828 · 640 bp

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Pi 0.0126
MarkerSeek score 66.60
Primer available yes
Label trnI-GAU-rrn16

Feature Metadata

label_name
trnI-GAU-rrn16
feature_type
igs
region
Genome
length_bp
640
variable_sites
8
pi
0.0126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rrn16_p1 CGCTGATGAGCACATTGAAC CAAGGTAGCCGTACTGGAAG 50.0 55.0 57.8 57.7 775-779 bp 1.0 77.5
trnI-GAU-rrn16_p2 CGCTGATGAGCACATTGAAC AGTGACTGGAGTGAAGTCGT 50.0 50.0 57.8 58.3 797-801 bp 1.0 77.4
trnI-GAU-rrn16_p3 GGCACAACGACGCAATTATC CAAGGTAGCCGTACTGGAAG 50.0 55.0 58.2 57.7 724-728 bp 1.0 77.4
trnI-GAU-rrn16_p4 GGCACAACGACGCAATTATC AGTGACTGGAGTGAAGTCGT 50.0 50.0 58.2 58.3 746-750 bp 1.0 77.3
trnI-GAU-rrn16_p5 CGCTGATGAGCACATTGAAC AAGTCGTAACAAGGTAGCCG 50.0 50.0 57.8 57.6 784-788 bp 1.0 77.0