Hotspot

ycf1-rps15

Genome · 92970-93763 · 794 bp

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Pi 0.0252
MarkerSeek score 73.20
Primer available yes
Label ycf1-rps15

Feature Metadata

label_name
ycf1-rps15
feature_type
igs
region
Genome
length_bp
794
variable_sites
20
pi
0.0252
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rps15_p1 CACCATTTTCCTGCTTTGCA GCAAATGCTGGGTAAGTCCT 45.0 50.0 57.5 58.2 954-958 bp 1.0 76.2
ycf1-rps15_p2 TGCACCATTTTCCTGCTTTG GCAAATGCTGGGTAAGTCCT 45.0 50.0 57.5 58.2 956-960 bp 1.0 76.1
ycf1-rps15_p3 ACTTTGCACCATTTTCCTGC GCAAATGCTGGGTAAGTCCT 45.0 50.0 57.5 58.2 960-964 bp 1.0 76.1
ycf1-rps15_p4 CACCATTTTCCTGCTTTGCA TGCAAATGCTGGGTAAGTCC 45.0 50.0 57.5 58.5 955-959 bp 1.0 73.8
ycf1-rps15_p5 TGCACCATTTTCCTGCTTTG TGCAAATGCTGGGTAAGTCC 45.0 50.0 57.5 58.5 957-961 bp 1.0 73.8