Hotspot

psbE-petL

Genome · 64951-65899 · 949 bp

Back to Nageia

Pi 0.0138
MarkerSeek score 64.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
Genome
length_bp
949
variable_sites
13
pi
0.0138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ATAATGTCAGCAAAAGCGCG GGTGGAAATTAGCAGAGCCA 45.0 50.0 57.8 57.9 1044-1048 bp 1.0 79.1
psbE-petL_p2 TTCTCCCGTATTCCCAGACA GGTGGAAATTAGCAGAGCCA 50.0 50.0 57.8 57.9 1024-1028 bp 1.0 78.7
psbE-petL_p3 TCTCCCGTATTCCCAGACAT GGTGGAAATTAGCAGAGCCA 50.0 50.0 57.5 57.9 1023-1027 bp 1.0 76.8
psbE-petL_p4 ATAATGTCAGCAAAAGCGCG AGGTGGAAATTAGCAGAGCC 45.0 50.0 57.8 57.6 1045-1049 bp 1.0 76.8
psbE-petL_p5 TTCTCCCGTATTCCCAGACA AGGTGGAAATTAGCAGAGCC 50.0 50.0 57.8 57.6 1025-1029 bp 1.0 76.3