Hotspot

rbcL-trnR-CCG

Genome · 55855-56404 · 550 bp

Back to Nageia

Pi 0.0291
MarkerSeek score 70.20
Primer available yes
Label rbcL-trnR-CCG

Feature Metadata

label_name
rbcL-trnR-CCG
feature_type
igs
region
Genome
length_bp
550
variable_sites
16
pi
0.0291
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-trnR-CCG_p1 GTGATCCGTGAAGCTAGCAA AACCATGTGCTCTGATCCAC 50.0 50.0 58.0 57.9 683-684 bp 1.0 80.8
rbcL-trnR-CCG_p2 GTGATCCGTGAAGCTAGCAA TCGGGCTAGAAGGGATTTGA 50.0 50.0 58.0 58.1 724-725 bp 1.0 80.7
rbcL-trnR-CCG_p3 GTGATCCGTGAAGCTAGCAA CTCGGGCTAGAAGGGATTTG 50.0 55.0 58.0 57.8 725-726 bp 1.0 79.7
rbcL-trnR-CCG_p4 GTGATCCGTGAAGCTAGCAA ATTGAATAGGGCTCGGGCTA 50.0 50.0 58.0 58.3 736-737 bp 1.0 79.5
rbcL-trnR-CCG_p5 GTGATCCGTGAAGCTAGCAA GATCCACTGAGCTACCAACC 50.0 55.0 58.0 57.7 670-671 bp 1.0 79.3