Hotspot

ycf3-trnS-GGA

Genome · 44441-45085 · 645 bp

Back to Nageia

Pi 0.0078
MarkerSeek score 63.80
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
Genome
length_bp
645
variable_sites
5
pi
0.0078
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCCGCTACAATTGTGAACGT ACGGAAAGAGAGGGATTCGA 45.0 50.0 57.8 58.2 788-792 bp 1.0 78.4
ycf3-trnS-GGA_p2 TCCGCTACAATTGTGAACGT TTCCAATGCTACGCCTTGAA 45.0 45.0 57.8 57.8 738-742 bp 1.0 78.2
ycf3-trnS-GGA_p3 CTCTTTTTCCCCTGAAGCCA ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.7 58.2 831-835 bp 1.0 77.3
ycf3-trnS-GGA_p4 TGCCTCTTTTTCCCCTGAAG ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.7 58.2 834-838 bp 1.0 77.3
ycf3-trnS-GGA_p5 CTCTTTTTCCCCTGAAGCCA TTCCAATGCTACGCCTTGAA 50.0 45.0 57.7 57.8 781-785 bp 1.0 77.0