Hotspot

atpA-atpF

Genome · 13103-13219 · 117 bp

Back to Nageia

Pi 0.0177
MarkerSeek score 65.90
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
Genome
length_bp
117
variable_sites
2
pi
0.0177
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 CATCGGGGCGAATAGTTACC AAAGAGCTCTGCGAACTCTG 55.0 50.0 58.2 57.9 251-255 bp 1.0 79.6
atpA-atpF_p2 CATCGGGGCGAATAGTTACC CAAAGAGCTCTGCGAACTCT 55.0 50.0 58.2 57.9 252-256 bp 1.0 79.6
atpA-atpF_p3 CATCGGGGCGAATAGTTACC CGATCATCATATCGGCCTCC 55.0 55.0 58.2 57.8 193-197 bp 1.0 79.1
atpA-atpF_p4 CATCGGGGCGAATAGTTACC GAGCTCTGCGAACTCTGAAT 55.0 50.0 58.2 57.7 248-252 bp 1.0 77.9
atpA-atpF_p5 CATCGGGGCGAATAGTTACC ATATCGGCCTCCTTAGAGCC 55.0 55.0 58.2 58.4 185-189 bp 1.0 77.4