Hotspot

trnK-UUU-chlB

Genome · 4058-5938 · 1881 bp

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Pi 0.0169
MarkerSeek score 72.20
Primer available yes
Label trnK-UUU-chlB

Feature Metadata

label_name
trnK-UUU-chlB
feature_type
igs
region
Genome
length_bp
1881
variable_sites
30
pi
0.0169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-chlB_p1 CGGGGGTATGGACGAATTTT AATTTGCACGACAGAAGGGT 50.0 45.0 58.0 58.0 1951-2052 bp 1.0 84.7
trnK-UUU-chlB_p2 TCTTACCAACTCTACCGGGG AATTTGCACGACAGAAGGGT 55.0 45.0 58.1 58.0 1966-2067 bp 1.0 83.9
trnK-UUU-chlB_p3 CGGGGGTATGGACGAATTTT TCCACGTTTTGTCAGGGAAA 50.0 45.0 58.0 57.6 1992-2093 bp 1.0 81.4
trnK-UUU-chlB_p4 CGGGGGTATGGACGAATTTT TTCCACGTTTTGTCAGGGAA 50.0 45.0 58.0 57.6 1993-2094 bp 1.0 81.4
trnK-UUU-chlB_p5 TCTTACCAACTCTACCGGGG TCCACGTTTTGTCAGGGAAA 55.0 45.0 58.1 57.6 2007-2108 bp 1.0 80.6