Hotspot

ycf1

SSC · 125886-131456 · 5571 bp

Back to Myrsine

Pi 0.0121

MarkerSeek score 46.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5571
variable_sites: 131
pi: 0.0121
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCACCTTTGATCTAAGATCTCT	GGTTTATACCAAACAACTTCCT	39.1	36.4	57.1	54.3	181 bp	1.0	46.2
ycf1_p2	GGTTTTGCACCTTTGATCTAAGA	GGTTTATACCAAACAACTTCCT	39.1	36.4	57.3	54.3	186 bp	1.0	46.0
ycf1_p3	GGTTTTGCACCTTTGATCTAAG	GGTTTATACCAAACAACTTCCT	40.9	36.4	55.9	54.3	186 bp	1.0	46.0
ycf1_p4	GGTTTTGCACCTTTGATCTA	GGTTTATACCAAACAACTTCCT	40.0	36.4	53.8	54.3	186 bp	1.0	46.0
ycf1_p5	GGTTTTGCACCTTTGATCTAAGA	GGTTTATACCAAACAACTTCC	39.1	38.1	57.3	52.7	186 bp	1.0	46.0