Hotspot

rpl16-rps3

LSC · 84552-84716 · 165 bp

Back to Myrsine

Pi 0.0646

MarkerSeek score 76.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 21
pi: 0.0646
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TTTCTCGTTTCCTTTCGCCA	TAGTAGCGGGGAAGTATGGG	45.0	55.0	58.0	58.0	1421-1530 bp	1.0	81.8
rpl16-rps3_p2	TTCATGGGTTCCATCGTTCC	TAGTAGCGGGGAAGTATGGG	50.0	55.0	57.9	58.0	1353-1462 bp	1.0	81.3
rpl16-rps3_p3	CGCGGGCGAATATTTACTCT	TAGTAGCGGGGAAGTATGGG	50.0	55.0	58.2	58.0	1500-1609 bp	1.0	80.9
rpl16-rps3_p4	TTCTCGTTTCCTTTCGCCAT	TAGTAGCGGGGAAGTATGGG	45.0	55.0	57.8	58.0	1420-1529 bp	1.0	80.7
rpl16-rps3_p5	TTCCATCGTTCCCATTGCTT	TAGTAGCGGGGAAGTATGGG	45.0	55.0	57.8	58.0	1345-1454 bp	1.0	80.6