Hotspot

psaJ-rpl33

LSC · 68748-69266 · 519 bp

Back to Myrsine

Pi 0.0225

MarkerSeek score 63.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 23
pi: 0.0225
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	CTCGGACATCTTTCCCCTTG	45.0	55.0	58.1	58.0	576-585 bp	1.0	82.6
psaJ-rpl33_p2	TGTGGCACCGGTACTAAGTA	CTCGGACATCTTTCCCCTTG	50.0	55.0	58.1	58.0	653-662 bp	1.0	82.2
psaJ-rpl33_p3	CATATCTCTCTGTGGCACCG	CTCGGACATCTTTCCCCTTG	55.0	55.0	57.9	58.0	663-672 bp	1.0	81.6
psaJ-rpl33_p4	ATGCGTTGACATTCCCCTTT	ACCGTTTCGACAACTGGTAC	45.0	50.0	58.1	58.1	614-623 bp	1.0	81.5
psaJ-rpl33_p5	ATGCGTTGACATTCCCCTTT	CCGTTTCGACAACTGGTACA	45.0	50.0	58.1	58.1	613-622 bp	1.0	81.5