Hotspot

petA-psbJ

LSC · 64675-65325 · 651 bp

Back to Myrsine

Pi 0.0297

MarkerSeek score 71.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 37
pi: 0.0297
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAAAAGGTTCAATTGTCCGA	GCCGATACTACTGGAAGGAT	42.9	50.0	57.1	56.2	801-806 bp	1.0	55.5
petA-psbJ_p2	TCGAAAAGGTTCAATTGTCCG	GCCGATACTACTGGAAGGAT	42.9	50.0	57.1	56.2	802-807 bp	1.0	55.5
petA-psbJ_p3	CGAAAAGGTTCAATTGTCCGA	GCCGATACTACTGGAAGGATT	42.9	47.6	57.1	57.0	801-806 bp	1.0	53.6
petA-psbJ_p4	TCGAAAAGGTTCAATTGTCCG	GCCGATACTACTGGAAGGATT	42.9	47.6	57.1	57.0	802-807 bp	1.0	53.6
petA-psbJ_p5	CGAAAAGGTTCAATTGTCCGA	GCCGATACTACTGGAAGGATTC	42.9	50.0	57.1	58.1	801-806 bp	1.0	53.2