Hotspot

psaI-ycf4

LSC · 60907-61329 · 423 bp

Back to Myrsine

Pi 0.011

MarkerSeek score 62.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 423
variable_sites: 9
pi: 0.011
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TAGGCCTAGTATTTCCGGCA	AAAAGGATAATGGCCCAGCA	50.0	45.0	57.9	57.5	571-593 bp	1.0	77.4
psaI-ycf4_p2	TAGGCCTAGTATTTCCGGCA	AAAGGATAATGGCCCAGCAA	50.0	45.0	57.9	57.5	570-592 bp	1.0	77.4
psaI-ycf4_p3	TAGGCCTAGTATTTCCGGCA	AAGGATAATGGCCCAGCAAA	50.0	45.0	57.9	57.5	569-591 bp	1.0	77.4
psaI-ycf4_p4	TAGGCCTAGTATTTCCGGCA	AGGATAATGGCCCAGCAAAA	50.0	45.0	57.9	57.5	568-590 bp	1.0	77.4
psaI-ycf4_p5	GCAATGGCAATGGCTTCTTT	AGGATAATGGCCCAGCAAAA	45.0	45.0	57.9	57.5	551-573 bp	1.0	77.3