Hotspot

trnF-GAA-ndhJ

LSC · 49727-50443 · 717 bp

Back to Myrsine

Pi 0.0126

MarkerSeek score 64.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 18
pi: 0.0126
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCGCGCTTGAAACGTATA	55.0	45.0	58.0	57.8	892-899 bp	1.0	80.0
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	TCCGCGCTTGAAACGTATAT	55.0	45.0	58.0	57.8	891-898 bp	1.0	80.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCCGCGCTTGAAACGTATA	50.0	45.0	58.1	57.8	856-863 bp	1.0	79.7
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TCCGCGCTTGAAACGTATAT	50.0	45.0	58.1	57.8	855-862 bp	1.0	79.7
trnF-GAA-ndhJ_p5	TCAAATCTGGTTCCTGGCAC	TCCGCGCTTGAAACGTATAT	50.0	45.0	58.1	57.8	840-847 bp	1.0	79.4