Hotspot

trnE-UUC-trnT-GGU

LSC · 31995-32865 · 871 bp

Back to Myrsine

Pi 0.00976

MarkerSeek score 69.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 16
pi: 0.00976
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	981-1048 bp	1.0	82.9
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	960-1027 bp	1.0	82.5
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	957-1024 bp	1.0	82.3
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	978-1045 bp	1.0	81.7
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	TTGAACCGATGACTTACGCC	55.0	50.0	57.8	58.3	983-1050 bp	1.0	80.7