Hotspot

rpoC1-rpoB

LSC · 24344-24370 · 27 bp

Back to Myrsine

Pi 0.037

MarkerSeek score 82.80

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 27
variable_sites: 2
pi: 0.037
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TGGCCCAAGCACTTATTTGT	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.0	57.5	219-221 bp	1.0	76.6
rpoC1-rpoB_p2	GATTTTCTTGGCCCAAGCAC	CCTGAGGATGCTCCAGAATC	50.0	55.0	57.9	57.5	227-229 bp	1.0	76.0
rpoC1-rpoB_p3	ATTTTCTTGGCCCAAGCACT	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.3	57.5	226-228 bp	1.0	74.4
rpoC1-rpoB_p4	GGGTTTTGTCACCTCTCCAA	CCTGAGGATGCTCCAGAATC	50.0	55.0	57.6	57.5	266-268 bp	1.0	73.7
rpoC1-rpoB_p5	TTTCTTGGCCCAAGCACTTA	CCTGAGGATGCTCCAGAATC	45.0	55.0	57.6	57.5	224-226 bp	1.0	73.7