Hotspot

atpH-atpI

LSC · 14588-15330 · 743 bp

Back to Myrsine

Pi 0.00999

MarkerSeek score 66.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 14
pi: 0.00999
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCTG	45.0	45.0	58.0	57.9	823-834 bp	1.0	80.9
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCCGCTG	45.0	45.0	57.9	57.9	815-826 bp	1.0	80.7
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCTG	50.0	45.0	57.9	57.9	879-890 bp	1.0	80.1
atpH-atpI_p4	AAGCGGCAGAAATCAATGGA	TTTTGCAACTTTAGCCGCTG	45.0	45.0	58.2	57.9	816-827 bp	1.0	79.9
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCCGCTG	45.0	45.0	58.2	57.9	824-835 bp	1.0	79.7