Hotspot

atpF-atpH

LSC · 13955-14341 · 387 bp

Back to Myrsine

Pi 0.0103

MarkerSeek score 63.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 8
pi: 0.0103
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTAGCCGAAAGAAACGA	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	536-559 bp	1.0	76.1
atpF-atpH_p2	CCAGTAGCCGAAAGAAACGA	CGAGGCGGAGGGAAAAATAC	50.0	55.0	57.9	58.7	541-564 bp	1.0	76.0
atpF-atpH_p3	CCAGTAGCCGAAAGAAACGA	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	540-563 bp	1.0	76.0
atpF-atpH_p4	CCAGTAGCCGAAAGAAACGA	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	537-560 bp	1.0	76.0
atpF-atpH_p5	CCAGTAGCCGAAAGAAACGA	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	538-561 bp	1.0	76.0