Hotspot

ycf1

SSC · 117918-123392 · 5475 bp

Back to Myrospermum

Pi 0.41

MarkerSeek score 74.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5475
variable_sites: 1887
pi: 0.41
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTCGGGTTATGGAAGAAGG	CACGATAGGGTCCGTTCAAG	55.0	55.0	58.0	58.1	1234 bp	0.5	59.0
ycf1_p2	ATGGAAGAAGGGACCGAGAA	CACGATAGGGTCCGTTCAAG	50.0	55.0	58.0	58.1	1225 bp	0.5	59.0
ycf1_p3	GCTCGGGTTATGGAAGAAGG	ACGATAGGGTCCGTTCAAGA	55.0	50.0	58.0	58.2	1233 bp	0.5	58.4
ycf1_p4	ATGGAAGAAGGGACCGAGAA	ACGATAGGGTCCGTTCAAGA	50.0	50.0	58.0	58.2	1224 bp	0.5	58.4
ycf1_p5	TCTCTTCCTTCTCCGAGCTC	CACGATAGGGTCCGTTCAAG	55.0	55.0	58.2	58.1	1250 bp	0.5	57.4