Hotspot

ndhF

SSC · 135408-137651 · 2244 bp

Back to Myrospermum

Pi 0.473

MarkerSeek score 81.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 939
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCCCGTTTTGAATACTTTGCA	CTTGAACGGACCCTATCGTG	42.9	55.0	58.0	58.1	2635 bp	0.5	51.2
ndhF_p2	GCCCGTTTTGAATACTTTGCA	TCTTGAACGGACCCTATCGT	42.9	50.0	58.0	58.2	2636 bp	0.5	50.6
ndhF_p3	AGCCCGTTTTGAATACTTTGC	CTTGAACGGACCCTATCGTG	42.9	55.0	57.7	58.1	2636 bp	0.5	49.0
ndhF_p4	AGCCCGTTTTGAATACTTTGC	TCTTGAACGGACCCTATCGT	42.9	50.0	57.7	58.2	2637 bp	0.5	48.4
ndhF_p5	GCCCGTTTTGAATACTTTGCA	ATCCTTTCTTGAACGGACCC	42.9	50.0	58.0	57.5	2642 bp	0.5	47.9