Hotspot

ndhA

SSC · 125365-127750 · 2386 bp

Back to Myrospermum

Pi 0.471

MarkerSeek score 78.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2386
variable_sites: 952
pi: 0.471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AAGAGTTGAAGCCCCCAAAG	CTCGGACTGTTTGTTGACCA	50.0	50.0	58.0	58.1	2717 bp	0.5	59.6
ndhA_p2	CAGGCTCTGGAAGGAATTCC	CTCGGACTGTTTGTTGACCA	55.0	50.0	58.0	58.1	2872 bp	0.5	59.2
ndhA_p3	GGCGGACCGTATGAGAATTT	CTCGGACTGTTTGTTGACCA	50.0	50.0	58.1	58.1	2851 bp	0.5	59.2
ndhA_p4	AAGCCCCCAAAGGAGAATTG	CTCGGACTGTTTGTTGACCA	50.0	50.0	58.1	58.1	2709 bp	0.5	59.1
ndhA_p5	TTCGTCCACCGGGTTTTATC	CTCGGACTGTTTGTTGACCA	50.0	50.0	57.9	58.1	2640 bp	0.5	58.8