Hotspot

trnL-UAG-rpl32

SSC · 133207-134157 · 951 bp

Back to Myrospermum

Pi 0.412

MarkerSeek score 82.00

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 951
variable_sites: 351
pi: 0.412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	ATGGGATTGTAGAGCGGGTA	GCAATCTCTTTCTACGGGCA	50.0	50.0	57.9	58.0	1578 bp	0.5	59.0
trnL-UAG-rpl32_p2	ATCATGCCTTTTTCGGACGA	GCAATCTCTTTCTACGGGCA	45.0	50.0	57.9	58.0	1313 bp	0.5	58.9
trnL-UAG-rpl32_p3	TCATGCCTTTTTCGGACGAA	GCAATCTCTTTCTACGGGCA	45.0	50.0	58.1	58.0	1312 bp	0.5	58.9
trnL-UAG-rpl32_p4	TGCCTTTTTCGGACGAATCA	GCAATCTCTTTCTACGGGCA	45.0	50.0	58.1	58.0	1309 bp	0.5	58.9
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	GCAATCTCTTTCTACGGGCA	50.0	50.0	57.9	58.0	1048 bp	0.5	58.8