Hotspot

trnP-UGG-psaJ

LSC · 72950-73858 · 909 bp

Back to Myrospermum

Pi 0.102

MarkerSeek score 87.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 909
variable_sites: 86
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 87.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1028-1330 bp	1.0	87.7
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1027-1329 bp	1.0	87.7
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1039-1341 bp	1.0	87.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1038-1340 bp	1.0	87.6
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1041-1343 bp	1.0	87.5