Hotspot

ycf4-cemA

LSC · 66313-66792 · 480 bp

Back to Myrospermum

Pi 0.0719

MarkerSeek score 82.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 33
pi: 0.0719
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AATGCGAAAGAGATGCACCA	45.0	45.0	58.1	58.2	593-599 bp	1.0	85.1
ycf4-cemA_p2	AAAAAGCTGCAGAATTGGCC	AATGCGAAAGAGATGCACCA	45.0	45.0	57.8	58.2	607-613 bp	1.0	84.3
ycf4-cemA_p3	AAAGCTGCAGAATTGGCCTA	AATGCGAAAGAGATGCACCA	45.0	45.0	57.8	58.2	605-611 bp	1.0	83.9
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	GCACCAGGGCAAAAAGACTA	45.0	50.0	58.1	58.4	579-585 bp	1.0	83.6
ycf4-cemA_p5	GCCATTCCCTTGACTCGTAC	AATGCGAAAGAGATGCACCA	55.0	45.0	58.3	58.2	659-665 bp	1.0	82.4