Hotspot

ndhC-trnV-UAC

LSC · 56140-57153 · 1014 bp

Back to Myrospermum

Pi 0.224

MarkerSeek score 87.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 216
pi: 0.224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGCTCGATATTATTAGAAATGCCC	AGAAGGTCTACGGTTCGAGT	41.7	50.0	57.6	57.8	1102-1329 bp	1.0	54.7
ndhC-trnV-UAC_p2	GGCTCGATATTATTAGAAATGCCC	GAAGGTCTACGGTTCGAGTC	41.7	55.0	57.6	57.5	1101-1328 bp	1.0	51.9
ndhC-trnV-UAC_p3	GGCTCGATATTATTAGAAATGCCC	GTTCGAGTCCGTATAGCCCT	41.7	55.0	57.6	58.7	1090-1317 bp	1.0	51.2
ndhC-trnV-UAC_p4	GCTCGATATTATTAGAAATGCCCA	AGAAGGTCTACGGTTCGAGT	37.5	50.0	57.0	57.8	1101-1328 bp	1.0	51.2
ndhC-trnV-UAC_p5	GGCTCGATATTATTAGAAATGCCC	AAGGTCTACGGTTCGAGTCC	41.7	55.0	57.6	58.8	1100-1327 bp	1.0	51.2