primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGCCGAGTACTCTACCATTGA	AAGGCGGGTGTTTTTATGGA	21	20	47.6	45.0	58.0	57.7	20.3	0.0	1.310376	4474	6253	1811-1866	1.000000	81.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AGCCGAGTACTCTACCATTGA	AGGCGGGTGTTTTTATGGAA	21	20	47.6	45.0	58.0	57.7	20.3	0.0	1.310376	4474	6253	1810-1865	1.000000	81.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGCCGAGTACTCTACCATTGA	GGCGGGTGTTTTTATGGAAC	21	20	47.6	50.0	58.0	57.6	20.3	0.0	1.371631	4474	6253	1809-1864	1.000000	81.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GCCGAGTACTCTACCATTGAG	AAGGCGGGTGTTTTTATGGA	21	20	52.4	45.0	57.6	57.7	20.3	0.0	1.653238	4474	6253	1810-1865	1.000000	78.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	GCCGAGTACTCTACCATTGAG	AGGCGGGTGTTTTTATGGAA	21	20	52.4	45.0	57.6	57.7	20.3	0.0	1.653238	4474	6253	1809-1864	1.000000	78.8
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	12243	12693	491-531	1.000000	74.4
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	12243	12693	494-534	1.000000	74.1
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	12243	12693	497-537	1.000000	73.6
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	12243	12693	500-540	1.000000	73.4
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	ACCAAAGTTTCACGTTCGGT	AGAAGACCTCTGTCCTATCCA	20	21	45.0	47.6	58.3	56.9	0.0	0.0	2.310076	12243	12693	561-601	1.000000	71.7
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.0	57.8	0.0	1.1	0.185568	40809	41408	754-2450	1.000000	81.8
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	40809	41408	757-2453	1.000000	80.2
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.395214	40809	41408	750-2446	1.000000	80.1
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.3	57.8	0.0	1.1	0.425837	40809	41408	755-2451	1.000000	79.8
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.4	57.8	0.0	1.1	0.530110	40809	41408	753-2449	1.000000	79.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	51335	52764	1426-1480	1.000000	89.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	51335	52764	1434-1488	1.000000	88.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	51335	52764	1458-1512	1.000000	88.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	51335	52764	1466-1520	1.000000	87.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	51335	52764	1440-1494	1.000000	87.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGCTCGATATTATTAGAAATGCCC	AGAAGGTCTACGGTTCGAGT	24	20	41.7	50.0	57.6	57.8	0.0	0.0	4.562228	56140	57153	1102-1329	1.000000	54.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGCTCGATATTATTAGAAATGCCC	GAAGGTCTACGGTTCGAGTC	24	20	41.7	55.0	57.6	57.5	0.0	0.0	4.906000	56140	57153	1101-1328	1.000000	51.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGCTCGATATTATTAGAAATGCCC	GTTCGAGTCCGTATAGCCCT	24	20	41.7	55.0	57.6	58.7	0.0	0.0	5.048294	56140	57153	1090-1317	1.000000	51.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GCTCGATATTATTAGAAATGCCCA	AGAAGGTCTACGGTTCGAGT	24	20	37.5	50.0	57.0	57.8	0.0	0.0	5.182471	56140	57153	1101-1328	1.000000	51.2
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGCTCGATATTATTAGAAATGCCC	AAGGTCTACGGTTCGAGTCC	24	20	41.7	55.0	57.6	58.8	0.0	0.0	5.192770	56140	57153	1100-1327	1.000000	51.2
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	AATGCGAAAGAGATGCACCA	20	20	45.0	45.0	58.1	58.2	3.3	0.0	0.270623	66313	66792	593-599	1.000000	85.1
ycf4-cemA_p2	ycf4-cemA	2	AAAAAGCTGCAGAATTGGCC	AATGCGAAAGAGATGCACCA	20	20	45.0	45.0	57.8	58.2	20.4	0.0	0.349273	66313	66792	607-613	1.000000	84.3
ycf4-cemA_p3	ycf4-cemA	3	AAAGCTGCAGAATTGGCCTA	AATGCGAAAGAGATGCACCA	20	20	45.0	45.0	57.8	58.2	20.4	0.0	0.395562	66313	66792	605-611	1.000000	83.9
ycf4-cemA_p4	ycf4-cemA	4	TTGGCCTATTTCTTGCGTGT	GCACCAGGGCAAAAAGACTA	20	20	45.0	50.0	58.1	58.4	3.3	0.0	0.479555	66313	66792	579-585	1.000000	83.6
ycf4-cemA_p5	ycf4-cemA	5	GCCATTCCCTTGACTCGTAC	AATGCGAAAGAGATGCACCA	20	20	55.0	45.0	58.3	58.2	0.0	0.0	0.521758	66313	66792	659-665	1.000000	82.4
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.269767	72950	73858	1028-1330	1.000000	87.7
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.271110	72950	73858	1027-1329	1.000000	87.7
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.277803	72950	73858	1039-1341	1.000000	87.6
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.279136	72950	73858	1038-1340	1.000000	87.6
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.279136	72950	73858	1041-1343	1.000000	87.5
rps11-rpl36_p1	rps11-rpl36	1	TCGTTGCATACCTTGATCCG	GAATGCGTGAGGGGGTTTAA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.236432	86616	87486	1593-1733	1.000000	88.9
rps11-rpl36_p2	rps11-rpl36	2	ACTTCCACTGTAGTGTCCGA	GAATGCGTGAGGGGGTTTAA	20	20	50.0	50.0	58.0	58.2	10.8	0.0	0.179900	86616	87486	1866-2005	1.000000	88.2
rps11-rpl36_p3	rps11-rpl36	3	TTCCACTGTAGTGTCCGAGT	GAATGCGTGAGGGGGTTTAA	20	20	50.0	50.0	58.0	58.2	10.8	0.0	0.179900	86616	87486	1864-2003	1.000000	88.2
rps11-rpl36_p4	rps11-rpl36	4	GAAATCACCCGACCCAGTAC	GAATGCGTGAGGGGGTTTAA	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.190968	86616	87486	1478-1478	0.500000	58.5
rps11-rpl36_p5	rps11-rpl36	5	AGAAATCACCCGACCCAGTA	GAATGCGTGAGGGGGTTTAA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.224036	86616	87486	1479-1479	0.500000	58.2
rps8-rpl14_p1	rps8-rpl14	1	TTATGTCAGCGATGGTGTCC	TTTCATTAGCACCCGAGGTA	20	20	50.0	45.0	57.7	56.2	0.0	0.0	2.059785	88441	89177	777-791	1.000000	75.5
rps8-rpl14_p2	rps8-rpl14	2	GTCAGCGATGGTGTCCTTAC	TTTCATTAGCACCCGAGGTA	20	20	55.0	45.0	58.4	56.2	0.0	0.0	2.108681	88441	89177	773-787	1.000000	75.1
rps8-rpl14_p3	rps8-rpl14	3	TATGTCAGCGATGGTGTCCT	TTTCATTAGCACCCGAGGTA	20	20	50.0	45.0	58.5	56.2	0.0	0.0	2.265134	88441	89177	776-790	1.000000	73.9
rps8-rpl14_p4	rps8-rpl14	4	TTATGTCAGCGATGGTGTCC	TCATTAGCACCCGAGGTATT	20	20	50.0	45.0	57.7	56.0	0.0	0.0	2.294454	88441	89177	775-789	1.000000	73.6
rps8-rpl14_p5	rps8-rpl14	5	TTATGTCAGCGATGGTGTCC	TTCATTAGCACCCGAGGTAT	20	20	50.0	45.0	57.7	56.0	0.0	0.0	2.294454	88441	89177	776-790	1.000000	73.6
ycf1_p1	ycf1	1	GCTCGGGTTATGGAAGAAGG	CACGATAGGGTCCGTTCAAG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.122503	118709	118828	1234-1234	0.500000	59.0
ycf1_p2	ycf1	2	ATGGAAGAAGGGACCGAGAA	CACGATAGGGTCCGTTCAAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.124808	118709	118828	1225-1225	0.500000	59.0
ycf1_p3	ycf1	3	GCTCGGGTTATGGAAGAAGG	ACGATAGGGTCCGTTCAAGA	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.201705	118709	118828	1233-1233	0.500000	58.4
ycf1_p4	ycf1	4	ATGGAAGAAGGGACCGAGAA	ACGATAGGGTCCGTTCAAGA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.204010	118709	118828	1224-1224	0.500000	58.4
ycf1_p5	ycf1	5	TCTCTTCCTTCTCCGAGCTC	CACGATAGGGTCCGTTCAAG	20	20	55.0	55.0	58.2	58.1	2.7	0.0	0.319747	118709	118828	1250-1250	0.500000	57.4
ndhH_p1	ndhH	1	CCGGGATCCGTTGAATTTCA	TGTCTTAACGAATCGCACGT	20	20	50.0	45.0	58.3	57.9	5.5	1.2	0.309455	124182	125363	2077-2077	0.500000	57.5
ndhH_p2	ndhH	2	GGGGGAGGGGCTTTAATTTT	TGTCTTAACGAATCGCACGT	20	20	50.0	45.0	57.7	57.9	0.0	1.2	0.318582	124182	125363	2151-2151	0.500000	57.5
ndhH_p3	ndhH	3	CCGGGATCCGTTGAATTTCA	TCAGGTCCAATACGCTGTTG	20	20	50.0	50.0	58.3	57.9	5.5	0.0	0.348074	124182	125363	1700-1700	0.500000	57.2
ndhH_p4	ndhH	4	GGGGGAGGGGCTTTAATTTT	TCAGGTCCAATACGCTGTTG	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.357201	124182	125363	1774-1774	0.500000	57.1
ndhH_p5	ndhH	5	TTCTGGGAAAACGTCAACGA	TGTCTTAACGAATCGCACGT	20	20	45.0	45.0	57.7	57.9	9.9	1.2	0.367410	124182	125363	1965-1965	0.500000	57.1
ndhA_p1	ndhA	1	AAGAGTTGAAGCCCCCAAAG	CTCGGACTGTTTGTTGACCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.052972	125365	127750	2717-2717	0.500000	59.6
ndhA_p2	ndhA	2	CAGGCTCTGGAAGGAATTCC	CTCGGACTGTTTGTTGACCA	20	20	55.0	50.0	58.0	58.1	17.3	0.0	0.101784	125365	127750	2872-2872	0.500000	59.2
ndhA_p3	ndhA	3	GGCGGACCGTATGAGAATTT	CTCGGACTGTTTGTTGACCA	20	20	50.0	50.0	58.1	58.1	16.7	0.0	0.102430	125365	127750	2851-2851	0.500000	59.2
ndhA_p4	ndhA	4	AAGCCCCCAAAGGAGAATTG	CTCGGACTGTTTGTTGACCA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.109119	125365	127750	2709-2709	0.500000	59.1
ndhA_p5	ndhA	5	TTCGTCCACCGGGTTTTATC	CTCGGACTGTTTGTTGACCA	20	20	50.0	50.0	57.9	58.1	6.3	0.0	0.149575	125365	127750	2640-2640	0.500000	58.8
ndhG_p1	ndhG	1	TGGTCAACAAACAGTCCGAG	GAAACAATAGCCGGTCCGAT	20	20	50.0	50.0	58.1	58.1	0.0	14.4	0.102430	128535	129065	1690-1690	0.500000	59.2
ndhG_p2	ndhG	2	AGGCCGTTTACCAATGTCAG	GAAACAATAGCCGGTCCGAT	20	20	50.0	50.0	58.2	58.1	0.0	14.4	0.229318	128535	129065	1297-1297	0.500000	58.2
ndhG_p3	ndhG	3	TGATTACCCTATCCCACGCA	GAAACAATAGCCGGTCCGAT	20	20	50.0	50.0	58.2	58.1	0.0	14.4	0.254518	128535	129065	1643-1643	0.500000	58.0
ndhG_p4	ndhG	4	TGCTTTAGGCCGTTTACCAA	GAAACAATAGCCGGTCCGAT	20	20	45.0	50.0	57.7	58.1	0.0	14.4	0.324073	128535	129065	1303-1303	0.500000	57.4
ndhG_p5	ndhG	5	TTGCTTTAGGCCGTTTACCA	GAAACAATAGCCGGTCCGAT	20	20	45.0	50.0	57.7	58.1	0.0	14.4	0.324073	128535	129065	1304-1304	0.500000	57.4
ndhD_p1	ndhD	1	AAATTGCTTCTGCCCCAAGA	GCTAATGAGGCATGGGGATC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.182430	130276	131775	2293-2293	0.500000	58.5
ndhD_p2	ndhD	2	AATTGCTTCTGCCCCAAGAA	GCTAATGAGGCATGGGGATC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.182430	130276	131775	2292-2292	0.500000	58.5
ndhD_p3	ndhD	3	AAACAACTCGAAGCATGGGT	GCTAATGAGGCATGGGGATC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.187163	130276	131775	2191-2191	0.500000	58.5
ndhD_p4	ndhD	4	GGCATGAAACAACTCGAAGC	GCTAATGAGGCATGGGGATC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.199316	130276	131775	2197-2197	0.500000	58.4
ndhD_p5	ndhD	5	AATTGCTTCTGCCCCAAGAA	GGGAGCAGTATGGGCTAATG	20	20	45.0	55.0	58.0	57.8	0.0	0.0	0.205137	130276	131775	2305-2305	0.500000	58.4
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	ATGGGATTGTAGAGCGGGTA	GCAATCTCTTTCTACGGGCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.121286	133207	134157	1578-1578	0.500000	59.0
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	ATCATGCCTTTTTCGGACGA	GCAATCTCTTTCTACGGGCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.132126	133207	134157	1313-1313	0.500000	58.9
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	TCATGCCTTTTTCGGACGAA	GCAATCTCTTTCTACGGGCA	20	20	45.0	50.0	58.1	58.0	2.6	0.0	0.138616	133207	134157	1312-1312	0.500000	58.9
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	TGCCTTTTTCGGACGAATCA	GCAATCTCTTTCTACGGGCA	20	20	45.0	50.0	58.1	58.0	8.1	0.0	0.138616	133207	134157	1309-1309	0.500000	58.9
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TCCTAAGAGCAGCGTGTCTA	GCAATCTCTTTCTACGGGCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.148453	133207	134157	1048-1048	0.500000	58.8
ndhF_p1	ndhF	1	GCCCGTTTTGAATACTTTGCA	CTTGAACGGACCCTATCGTG	21	20	42.9	55.0	58.0	58.1	0.0	0.0	1.097362	135408	137651	2635-2635	0.500000	51.2
ndhF_p2	ndhF	2	GCCCGTTTTGAATACTTTGCA	TCTTGAACGGACCCTATCGT	21	20	42.9	50.0	58.0	58.2	0.0	0.0	1.176564	135408	137651	2636-2636	0.500000	50.6
ndhF_p3	ndhF	3	AGCCCGTTTTGAATACTTTGC	CTTGAACGGACCCTATCGTG	21	20	42.9	55.0	57.7	58.1	0.0	0.0	1.375260	135408	137651	2636-2636	0.500000	49.0
ndhF_p4	ndhF	4	AGCCCGTTTTGAATACTTTGC	TCTTGAACGGACCCTATCGT	21	20	42.9	50.0	57.7	58.2	0.0	0.0	1.454462	135408	137651	2637-2637	0.500000	48.4
ndhF_p5	ndhF	5	GCCCGTTTTGAATACTTTGCA	ATCCTTTCTTGAACGGACCC	21	20	42.9	50.0	58.0	57.5	0.0	10.2	1.511608	135408	137651	2642-2642	0.500000	47.9