Hotspot

trnN-GUU-trnR-ACG

IRa · 139806-140449 · 644 bp

Back to Myrocarpus

Pi 0.011

MarkerSeek score 65.80

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 644
variable_sites: 7
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	780-785 bp	1.0	79.5
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	817-822 bp	1.0	78.9
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	TTTTAGGGTACTCCGGGGAA	55.0	50.0	58.0	57.7	834-839 bp	1.0	78.4
trnN-GUU-trnR-ACG_p4	CCGTTGTTCCTCAGTAGCTC	TTTTTAGGGTACTCCGGGGA	55.0	50.0	58.0	57.7	835-840 bp	1.0	78.4
trnN-GUU-trnR-ACG_p5	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	744-749 bp	1.0	78.4