Hotspot

ndhD-psaC

SSC · 126788-126917 · 130 bp

Back to Myrocarpus

Pi 0.0388

MarkerSeek score 66.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 130
variable_sites: 5
pi: 0.0388
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGATTAAGGAACCCCCAGCT	AAACAACTCGAAGCATGGGT	50.0	45.0	58.0	58.0	225-226 bp	1.0	81.9
ndhD-psaC_p2	AGATTAAGGAACCCCCAGCT	GGCATGAAACAACTCGAAGC	50.0	50.0	58.0	58.0	231-232 bp	1.0	81.7
ndhD-psaC_p3	CCCCAGCTATTGGCAAAACT	AAACAACTCGAAGCATGGGT	50.0	45.0	58.4	58.0	213-214 bp	1.0	78.6
ndhD-psaC_p4	CCCCAGCTATTGGCAAAACT	GGCATGAAACAACTCGAAGC	50.0	50.0	58.4	58.0	219-220 bp	1.0	78.4
ndhD-psaC_p5	AGATTAAGGAACCCCCAGCT	AACAACTCGAAGCATGGGTC	50.0	50.0	58.0	58.5	224-225 bp	1.0	78.2