Hotspot

trnR-ACG-trnN-GUU

IRb · 117480-118123 · 644 bp

Back to Myrocarpus

Pi 0.011

MarkerSeek score 65.80

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 644
variable_sites: 7
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	780-785 bp	1.0	79.5
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	817-822 bp	1.0	78.9
trnR-ACG-trnN-GUU_p3	TTTTAGGGTACTCCGGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	834-839 bp	1.0	78.4
trnR-ACG-trnN-GUU_p4	TTTTTAGGGTACTCCGGGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	835-840 bp	1.0	78.4
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	744-749 bp	1.0	78.4