Hotspot

psbH-petB

LSC · 83059-83204 · 146 bp

Back to Myrocarpus

Pi 0.0274

MarkerSeek score 67.80

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 146
variable_sites: 4
pi: 0.0274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	TCCTTTGATGGGTGTTGCAA	CGACGGGGTAAAACCATCTC	45.0	55.0	57.9	58.4	340 bp	1.0	77.5
psbH-petB_p2	TCCTTTGATGGGTGTTGCAA	TATCCGACGGGGTAAAACCA	45.0	50.0	57.9	58.4	344 bp	1.0	76.8
psbH-petB_p3	GGGTGTTGCAATGGCTCTAT	CGACGGGGTAAAACCATCTC	50.0	55.0	58.2	58.4	331 bp	1.0	76.3
psbH-petB_p4	TGCAATGGCTCTATTTGCGA	CGACGGGGTAAAACCATCTC	45.0	55.0	58.3	58.4	325 bp	1.0	76.2
psbH-petB_p5	GGGTGTTGCAATGGCTCTAT	TATCCGACGGGGTAAAACCA	50.0	50.0	58.2	58.4	335 bp	1.0	75.6