Hotspot

trnP-UGG-psaJ

LSC · 73821-75048 · 1228 bp

Back to Myrocarpus

Pi 0.0234

MarkerSeek score 76.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 1228
variable_sites: 27
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1347-1373 bp	1.0	83.4
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1346-1372 bp	1.0	83.4
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1358-1384 bp	1.0	83.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1357-1383 bp	1.0	83.3
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	1360-1386 bp	1.0	83.3