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psbE-petL

LSC · 71489-73017 · 1529 bp

Back to Myrocarpus

Pi 0.0201

MarkerSeek score 62.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1529
variable_sites: 30
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGTCGCTAGTAGAAAACCGA	45.0	45.0	58.0	56.0	1686-1700 bp	1.0	67.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGTCGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.0	1675-1689 bp	1.0	67.0
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGTCGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.0	1598-1612 bp	1.0	66.3
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGTCGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.0	1593-1607 bp	1.0	66.0
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGTCGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.0	1677-1691 bp	1.0	65.5