Hotspot

trnS-GCU-trnG-UCC

LSC · 10511-11812 · 1302 bp

Back to Myrocarpus

Pi 0.0421
MarkerSeek score 72.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1302
variable_sites
46
pi
0.0421
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGGGTCCATAGAACGAATCG 50.0 55.0 58.0 57.8 1278-1383 bp 1.0 85.5
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC GGGGTCCATAGAACGAATCG 50.0 55.0 58.0 57.8 1277-1382 bp 1.0 85.5
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA GGTCCATAGAACGAATCGCA 50.0 50.0 58.0 57.8 1276-1381 bp 1.0 85.0
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GGTCCATAGAACGAATCGCA 50.0 50.0 58.0 57.8 1275-1380 bp 1.0 85.0
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA GGGGTCCATAGAACGAATCG 50.0 55.0 58.2 57.8 1331-1436 bp 1.0 84.2