primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 20 20 55.0 55.0 58.1 57.9 0.0 0.0 0.203648 120 520 470-500 1.000000 82.3 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 GATCCACTTGGCTACATCCG TAACCGGGCTAACCTTGGTA 20 20 55.0 50.0 58.1 58.1 0.0 6.3 0.179121 120 520 522-552 1.000000 82.1 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 CAATCCACTGCCTTGATCCA TAACCGGGCTAACCTTGGTA 20 20 50.0 50.0 57.9 58.1 0.0 6.3 0.183482 120 520 536-566 1.000000 82.0 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 AATCCACTGCCTTGATCCAC TAACCGGGCTAACCTTGGTA 20 20 50.0 50.0 57.9 58.1 0.0 6.3 0.184990 120 520 535-565 1.000000 82.0 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 ACAATCCACTGCCTTGATCC TAACCGGGCTAACCTTGGTA 20 20 50.0 50.0 57.9 58.1 0.0 6.3 0.184990 120 520 537-567 1.000000 82.0 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 AGCCGAGTACTCTACCATTGA AAGGCGGGTGTTTTTATGGA 21 20 47.6 45.0 58.0 57.7 20.3 0.0 1.310376 4473 6210 1824-2189 1.000000 73.3 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 AGCCGAGTACTCTACCATTGA AGGCGGGTGTTTTTATGGAA 21 20 47.6 45.0 58.0 57.7 20.3 0.0 1.310376 4473 6210 1823-2188 1.000000 73.3 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 AGCCGAGTACTCTACCATTGA GGCGGGTGTTTTTATGGAAC 21 20 47.6 50.0 58.0 57.6 20.3 0.0 1.371631 4473 6210 1822-2187 1.000000 72.8 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 GCCGAGTACTCTACCATTGAG AAGGCGGGTGTTTTTATGGA 21 20 52.4 45.0 57.6 57.7 20.3 0.0 1.653238 4473 6210 1823-2188 1.000000 70.6 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 GCCGAGTACTCTACCATTGAG AGGCGGGTGTTTTTATGGAA 21 20 52.4 45.0 57.6 57.7 20.3 0.0 1.653238 4473 6210 1822-2187 1.000000 70.6 trnQ-UUG-psbK_p1 trnQ-UUG-psbK 1 CTTCGAATAGCGGGACCAAA ACTTGAATAAAGGGCAGAGTGA 20 22 50.0 40.9 58.0 57.4 0.0 0.0 2.659313 8108 9500 1501-1590 1.000000 64.4 trnQ-UUG-psbK_p2 trnQ-UUG-psbK 2 TCGAATAGCGGGACCAAAAC ACTTGAATAAAGGGCAGAGTGA 20 22 50.0 40.9 58.3 57.4 0.0 0.0 2.911488 8108 9500 1499-1588 1.000000 62.4 trnQ-UUG-psbK_p3 trnQ-UUG-psbK 3 CTTCGAATAGCGGGACCAAA ACTTGAATAAAGGGCAGAGTGAA 20 23 50.0 39.1 58.0 58.0 0.0 0.0 3.033004 8108 9500 1501-1590 1.000000 61.4 trnQ-UUG-psbK_p4 trnQ-UUG-psbK 4 GAACCTTCGAATAGCGGGAC ACTTGAATAAAGGGCAGAGTGA 20 22 55.0 40.9 58.4 57.4 7.3 0.0 3.072166 8108 9500 1505-1594 1.000000 61.1 trnQ-UUG-psbK_p5 trnQ-UUG-psbK 5 TTCGAATAGCGGGACCAAAA ACTTGAATAAAGGGCAGAGTGA 20 22 45.0 40.9 57.5 57.4 0.0 0.0 3.116898 8108 9500 1500-1589 1.000000 60.7 trnS-GCU-trnG-UCC_p1 trnS-GCU-trnG-UCC 1 CAATCCGACGCTTTAGTCCA GGGGTCCATAGAACGAATCG 20 20 50.0 55.0 58.0 57.8 0.0 0.0 0.154980 10511 11812 1278-1383 1.000000 85.5 trnS-GCU-trnG-UCC_p2 trnS-GCU-trnG-UCC 2 AATCCGACGCTTTAGTCCAC GGGGTCCATAGAACGAATCG 20 20 50.0 55.0 58.0 57.8 0.0 0.0 0.156368 10511 11812 1277-1382 1.000000 85.5 trnS-GCU-trnG-UCC_p3 trnS-GCU-trnG-UCC 3 CAATCCGACGCTTTAGTCCA GGTCCATAGAACGAATCGCA 20 20 50.0 50.0 58.0 57.8 0.0 1.2 0.223536 10511 11812 1276-1381 1.000000 85.0 trnS-GCU-trnG-UCC_p4 trnS-GCU-trnG-UCC 4 AATCCGACGCTTTAGTCCAC GGTCCATAGAACGAATCGCA 20 20 50.0 50.0 58.0 57.8 0.0 1.2 0.224924 10511 11812 1275-1380 1.000000 85.0 trnS-GCU-trnG-UCC_p5 trnS-GCU-trnG-UCC 5 ACGGAAAGAGAGGGATTCGA GGGGTCCATAGAACGAATCG 20 20 50.0 55.0 58.2 57.8 0.0 0.0 0.301746 10511 11812 1331-1436 1.000000 84.2 trnC-GCA-petN_p1 trnC-GCA-petN 1 TTTTGTATCGTTTTGGCGGC TTAAAGCAGCCCAAGCAAGA 20 20 45.0 45.0 57.9 58.0 0.0 0.0 0.059326 32318 32952 754-761 1.000000 81.7 trnC-GCA-petN_p2 trnC-GCA-petN 2 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.088172 32318 32952 698-704 1.000000 81.7 trnC-GCA-petN_p3 trnC-GCA-petN 3 TTTTGTATCGTTTTGGCGGC CCATACGACGAGTGAAAGGG 20 20 45.0 55.0 57.9 58.1 0.0 0.0 0.130712 32318 32952 792-799 1.000000 81.1 trnC-GCA-petN_p4 trnC-GCA-petN 4 TTTTGTATCGTTTTGGCGGC CCCATACGACGAGTGAAAGG 20 20 45.0 55.0 57.9 58.1 0.0 0.0 0.130712 32318 32952 793-800 1.000000 81.1 trnC-GCA-petN_p5 trnC-GCA-petN 5 TTTTGTATCGTTTTGGCGGC CCCCATACGACGAGTGAAAG 20 20 45.0 55.0 57.9 58.1 0.0 0.0 0.130712 32318 32952 794-801 1.000000 81.1 trnD-GUC-trnY-GUA_p1 trnD-GUC-trnY-GUA 1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 20 20 50.0 50.0 58.5 57.8 7.1 0.0 0.716274 34957 35416 530-532 1.000000 76.1 trnD-GUC-trnY-GUA_p2 trnD-GUC-trnY-GUA 2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 20 20 50.0 50.0 58.5 57.8 7.1 0.0 0.717825 34957 35416 529-531 1.000000 76.1 trnD-GUC-trnY-GUA_p3 trnD-GUC-trnY-GUA 3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 20 20 50.0 55.0 58.5 58.4 7.1 0.0 0.828828 34957 35416 551-553 1.000000 75.1 trnD-GUC-trnY-GUA_p4 trnD-GUC-trnY-GUA 4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 20 20 50.0 50.0 58.5 57.6 7.1 0.0 0.852505 34957 35416 550-552 1.000000 74.9 trnD-GUC-trnY-GUA_p5 trnD-GUC-trnY-GUA 5 CGGTGCTCTGACCAATTGAA GATTCTTCCTGGGTCGATGC 20 20 50.0 55.0 58.5 58.4 7.1 0.0 0.889785 34957 35416 584-586 1.000000 74.5 trnG-GCC-trnfM-CAU_p1 trnG-GCC-trnfM-CAU 1 TCTTTGCCAAGGAGAAGACG GTAGCTCGCAAGGCTCATAA 20 20 50.0 50.0 57.8 57.8 0.0 0.0 0.394901 42174 42388 314-419 1.000000 78.2 trnG-GCC-trnfM-CAU_p2 trnG-GCC-trnfM-CAU 2 TCTTTGCCAAGGAGAAGACG TAGCTCGCAAGGCTCATAAC 20 20 50.0 50.0 57.8 57.8 0.0 0.0 0.394901 42174 42388 313-418 1.000000 78.2 trnG-GCC-trnfM-CAU_p3 trnG-GCC-trnfM-CAU 3 TCTTTGCCAAGGAGAAGACG ATTCTTCTCTTTACCGCGGG 20 20 50.0 50.0 57.8 57.7 0.0 31.8 0.480956 42174 42388 347-452 1.000000 77.4 trnG-GCC-trnfM-CAU_p4 trnG-GCC-trnfM-CAU 4 TCTTTGCCAAGGAGAAGACG AGGTCATGGGTTCAAATCCC 20 20 50.0 50.0 57.8 57.5 0.0 0.0 0.702082 42174 42388 289-394 1.000000 75.8 trnG-GCC-trnfM-CAU_p5 trnG-GCC-trnfM-CAU 5 TCTTTGCCAAGGAGAAGACG CCTTGAGGTCATGGGTTCAA 20 20 50.0 50.0 57.8 57.4 0.0 4.3 0.754668 42174 42388 294-399 1.000000 75.3 trnV-UAC-trnM-CAU_p1 trnV-UAC-trnM-CAU 1 CGTGTAAACGAGGTGCTCTA CCCGCCGTATGAAAGCAATA 20 20 50.0 50.0 57.4 58.4 0.0 0.0 1.035642 58008 58187 263-264 1.000000 74.1 trnV-UAC-trnM-CAU_p2 trnV-UAC-trnM-CAU 2 CGTGTAAACGAGGTGCTCTA CCGCCGTATGAAAGCAATAC 20 20 50.0 50.0 57.4 57.3 0.0 0.0 1.287595 58008 58187 262-263 1.000000 72.1 trnV-UAC-trnM-CAU_p3 trnV-UAC-trnM-CAU 3 CGTGTAAACGAGGTGCTCTA TTTGAACCAATGACTCCCGC 20 20 50.0 50.0 57.4 58.8 0.0 0.0 1.380414 58008 58187 278-279 1.000000 71.3 trnV-UAC-trnM-CAU_p4 trnV-UAC-trnM-CAU 4 CGTGTAAACGAGGTGCTCTA GACTCCCGCCGTATGAAAG 20 19 50.0 57.9 57.4 58.0 0.0 0.0 1.650896 58008 58187 267-268 1.000000 69.2 trnV-UAC-trnM-CAU_p5 trnV-UAC-trnM-CAU 5 CGTGTAAACGAGGTGCTCTA TTGAACCAATGACTCCCGC 20 19 50.0 52.6 57.4 58.1 0.0 0.0 1.683106 58008 58187 277-278 1.000000 68.8 rbcL-accD_p1 rbcL-accD 1 AATGAGGGACGTGATCTTGC AATTCCGGATGTTCCTGACG 20 20 50.0 50.0 58.0 58.0 0.0 4.1 0.054014 62665 64227 1921-1968 1.000000 84.5 rbcL-accD_p2 rbcL-accD 2 TGGAGATCATGTTCACGCTG AATTCCGGATGTTCCTGACG 20 20 50.0 50.0 58.0 58.0 6.6 4.1 0.038511 62665 64227 2249-2296 1.000000 84.0 rbcL-accD_p3 rbcL-accD 3 GAGATCATGTTCACGCTGGT AATTCCGGATGTTCCTGACG 20 20 50.0 50.0 58.0 58.0 6.6 4.1 0.039911 62665 64227 2247-2294 1.000000 84.0 rbcL-accD_p4 rbcL-accD 4 GTGGAGATCATGTTCACGCT AATTCCGGATGTTCCTGACG 20 20 50.0 50.0 58.0 58.0 6.6 4.1 0.039911 62665 64227 2250-2297 1.000000 84.0 rbcL-accD_p5 rbcL-accD 5 ATCATGTTCACGCTGGTACC AATTCCGGATGTTCCTGACG 20 20 50.0 50.0 58.0 58.0 4.4 4.1 0.048351 62665 64227 2244-2291 1.000000 83.9 psbE-petL_p1 psbE-petL 1 TGACAAATAACCAACCCGCA AGTCGCTAGTAGAAAACCGA 20 20 45.0 45.0 58.0 56.0 0.0 0.0 1.984360 71489 73017 1686-1700 1.000000 67.2 psbE-petL_p2 psbE-petL 2 CAACCCGCAATGAATAGGGA AGTCGCTAGTAGAAAACCGA 20 20 50.0 45.0 57.9 56.0 6.4 0.0 2.018355 71489 73017 1675-1689 1.000000 67.0 psbE-petL_p3 psbE-petL 3 AGAACGTTCTCCTGTGCTTC AGTCGCTAGTAGAAAACCGA 20 20 50.0 45.0 57.8 56.0 24.9 0.0 2.123364 71489 73017 1598-1612 1.000000 66.3 psbE-petL_p4 psbE-petL 4 GTTCTCCTGTGCTTCCAGAC AGTCGCTAGTAGAAAACCGA 20 20 55.0 45.0 58.2 56.0 0.9 0.0 2.164952 71489 73017 1593-1607 1.000000 66.0 psbE-petL_p5 psbE-petL 5 ACCAACCCGCAATGAATAGG AGTCGCTAGTAGAAAACCGA 20 20 50.0 45.0 58.2 56.0 0.0 0.0 2.204313 71489 73017 1677-1691 1.000000 65.5 trnP-UGG-psaJ_p1 trnP-UGG-psaJ 1 ACCCGTGACATTTTGTACCC AAGCCCCGAACCATAGAGTA 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.269767 73821 75048 1347-1373 1.000000 83.4 trnP-UGG-psaJ_p2 trnP-UGG-psaJ 2 CCCGTGACATTTTGTACCCA AAGCCCCGAACCATAGAGTA 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.271110 73821 75048 1346-1372 1.000000 83.4 trnP-UGG-psaJ_p3 trnP-UGG-psaJ 3 ACAGGATTTGAACCCGTGAC AAGCCCCGAACCATAGAGTA 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.277803 73821 75048 1358-1384 1.000000 83.4 trnP-UGG-psaJ_p4 trnP-UGG-psaJ 4 CAGGATTTGAACCCGTGACA AAGCCCCGAACCATAGAGTA 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.279136 73821 75048 1357-1383 1.000000 83.3 trnP-UGG-psaJ_p5 trnP-UGG-psaJ 5 TGACAGGATTTGAACCCGTG AAGCCCCGAACCATAGAGTA 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.279136 73821 75048 1360-1386 1.000000 83.3 psbH-petB_p1 psbH-petB 1 TCCTTTGATGGGTGTTGCAA CGACGGGGTAAAACCATCTC 20 20 45.0 55.0 57.9 58.4 0.0 0.0 0.430666 83059 83204 340-340 1.000000 77.5 psbH-petB_p2 psbH-petB 2 TCCTTTGATGGGTGTTGCAA TATCCGACGGGGTAAAACCA 20 20 45.0 50.0 57.9 58.4 0.0 15.2 0.512959 83059 83204 344-344 1.000000 76.8 psbH-petB_p3 psbH-petB 3 GGGTGTTGCAATGGCTCTAT CGACGGGGTAAAACCATCTC 20 20 50.0 55.0 58.2 58.4 0.6 0.0 0.583737 83059 83204 331-331 1.000000 76.3 psbH-petB_p4 psbH-petB 4 TGCAATGGCTCTATTTGCGA CGACGGGGTAAAACCATCTC 20 20 45.0 55.0 58.3 58.4 0.0 0.0 0.602546 83059 83204 325-325 1.000000 76.2 psbH-petB_p5 psbH-petB 5 GGGTGTTGCAATGGCTCTAT TATCCGACGGGGTAAAACCA 20 20 50.0 50.0 58.2 58.4 0.6 15.2 0.666030 83059 83204 335-335 1.000000 75.6 trnR-ACG-trnN-GUU_p1 trnR-ACG-trnN-GUU 1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 20 20 55.0 55.0 57.8 58.0 18.9 0.0 0.218238 117480 118123 780-785 1.000000 79.5 trnR-ACG-trnN-GUU_p2 trnR-ACG-trnN-GUU 2 GAACAGATCCAGTGGAGACG CCGTTGTTCCTCAGTAGCTC 20 20 55.0 55.0 57.7 58.0 18.7 0.0 0.288158 117480 118123 817-822 1.000000 78.9 trnR-ACG-trnN-GUU_p3 trnR-ACG-trnN-GUU 3 TTTTAGGGTACTCCGGGGAA CCGTTGTTCCTCAGTAGCTC 20 20 50.0 55.0 57.7 58.0 9.3 0.0 0.343052 117480 118123 834-839 1.000000 78.4 trnR-ACG-trnN-GUU_p4 trnR-ACG-trnN-GUU 4 TTTTTAGGGTACTCCGGGGA CCGTTGTTCCTCAGTAGCTC 20 20 50.0 55.0 57.7 58.0 9.3 0.0 0.343052 117480 118123 835-840 1.000000 78.4 trnR-ACG-trnN-GUU_p5 trnR-ACG-trnN-GUU 5 CAGAGGATTAGAGCACGTGG GCTGTTAACTGACTGGTCGT 20 20 55.0 50.0 57.8 57.8 18.9 15.9 0.366304 117480 118123 744-749 1.000000 78.4 trnN-GUU-ndhF_p1 trnN-GUU-ndhF 1 ACGACCAGTCAGTTAACAGC GGGGGTGGACGAATTTCTTC 20 20 50.0 55.0 57.8 58.5 8.6 0.0 0.689893 118196 118683 621-674 1.000000 77.0 trnN-GUU-ndhF_p2 trnN-GUU-ndhF 2 AGGATTCGAACCTACGACCA GGGGGTGGACGAATTTCTTC 20 20 50.0 55.0 58.2 58.5 15.0 0.0 0.696128 118196 118683 634-687 1.000000 76.9 trnN-GUU-ndhF_p3 trnN-GUU-ndhF 3 GGATTCGAACCTACGACCAG GGGGGTGGACGAATTTCTTC 20 20 55.0 55.0 57.8 58.5 0.0 0.0 0.744803 118196 118683 633-686 1.000000 76.5 trnN-GUU-ndhF_p4 trnN-GUU-ndhF 4 CCCCAAGTAGGATTCGAACC GGGGGTGGACGAATTTCTTC 20 20 55.0 55.0 57.7 58.5 0.0 0.0 0.866696 118196 118683 642-695 1.000000 75.5 trnN-GUU-ndhF_p5 trnN-GUU-ndhF 5 TTCGAACCTACGACCAGTCA GGGGGTGGACGAATTTCTTC 20 20 50.0 55.0 58.4 58.5 0.0 0.0 0.931441 118196 118683 630-683 1.000000 75.0 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 GCAATCTCTTTCTACGGGCA TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.148453 122181 123841 1764-1931 1.000000 84.2 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AGCGCAATCTCTTTCTACGG TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 58.1 57.9 11.5 0.0 0.196382 122181 123841 1767-1934 1.000000 83.8 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 GCAATCTCTTTCTACGGGCA AGCAGCGTGTCTACCAATTT 20 20 50.0 45.0 58.0 57.8 0.0 0.0 0.209180 122181 123841 1757-1924 1.000000 83.7 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 GCAATCTCTTTCTACGGGCA CCTAAGAGCAGCGTGTCTAC 20 20 50.0 55.0 58.0 57.8 0.0 0.0 0.220471 122181 123841 1763-1930 1.000000 83.6 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 GCAATCTCTTTCTACGGGCA CTAAGAGCAGCGTGTCTACC 20 20 50.0 55.0 58.0 57.8 0.0 0.0 0.220471 122181 123841 1762-1929 1.000000 83.6 ndhD-psaC_p1 ndhD-psaC 1 AGATTAAGGAACCCCCAGCT AAACAACTCGAAGCATGGGT 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.031664 126788 126917 225-226 1.000000 81.9 ndhD-psaC_p2 ndhD-psaC 2 AGATTAAGGAACCCCCAGCT GGCATGAAACAACTCGAAGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.043817 126788 126917 231-232 1.000000 81.7 ndhD-psaC_p3 ndhD-psaC 3 CCCCAGCTATTGGCAAAACT AAACAACTCGAAGCATGGGT 20 20 50.0 45.0 58.4 58.0 3.0 0.0 0.458239 126788 126917 213-214 1.000000 78.6 ndhD-psaC_p4 ndhD-psaC 4 CCCCAGCTATTGGCAAAACT GGCATGAAACAACTCGAAGC 20 20 50.0 50.0 58.4 58.0 3.0 0.0 0.470392 126788 126917 219-220 1.000000 78.4 ndhD-psaC_p5 ndhD-psaC 5 AGATTAAGGAACCCCCAGCT AACAACTCGAAGCATGGGTC 20 20 50.0 50.0 58.0 58.5 0.0 0.0 0.489163 126788 126917 224-225 1.000000 78.2 trnN-GUU-trnR-ACG_p1 trnN-GUU-trnR-ACG 1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 20 20 55.0 55.0 58.0 57.8 0.0 18.9 0.218238 139806 140449 780-785 1.000000 79.5 trnN-GUU-trnR-ACG_p2 trnN-GUU-trnR-ACG 2 CCGTTGTTCCTCAGTAGCTC GAACAGATCCAGTGGAGACG 20 20 55.0 55.0 58.0 57.7 0.0 18.7 0.288158 139806 140449 817-822 1.000000 78.9 trnN-GUU-trnR-ACG_p3 trnN-GUU-trnR-ACG 3 CCGTTGTTCCTCAGTAGCTC TTTTAGGGTACTCCGGGGAA 20 20 55.0 50.0 58.0 57.7 0.0 9.3 0.343052 139806 140449 834-839 1.000000 78.4 trnN-GUU-trnR-ACG_p4 trnN-GUU-trnR-ACG 4 CCGTTGTTCCTCAGTAGCTC TTTTTAGGGTACTCCGGGGA 20 20 55.0 50.0 58.0 57.7 0.0 9.3 0.343052 139806 140449 835-840 1.000000 78.4 trnN-GUU-trnR-ACG_p5 trnN-GUU-trnR-ACG 5 GCTGTTAACTGACTGGTCGT CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 57.8 57.8 15.9 18.9 0.366304 139806 140449 744-749 1.000000 78.4