Hotspot

ycf1

SSC · 124983-129020 · 4038 bp

Back to Myristica

Pi 0.00267

MarkerSeek score 48.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4038
variable_sites: 24
pi: 0.00267
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGCCAAAGAAACAACTTG	TCGAAGATGCCGAGAGAAAA	45.0	45.0	57.0	57.0	317 bp	1.0	64.7
ycf1_p2	TGGGCCAAAGAAACAACTTG	ACTTGTGGATGGAGACGAAA	45.0	45.0	57.0	56.8	245 bp	1.0	63.4
ycf1_p3	TGGGCCAAAGAAACAACTTG	AATCGAAGATGCCGAGAGAA	45.0	45.0	57.0	56.7	319 bp	1.0	62.9
ycf1_p4	TGGGCCAAAGAAACAACTTG	ATCGAAGATGCCGAGAGAAA	45.0	45.0	57.0	56.7	318 bp	1.0	62.9
ycf1_p5	TTGGGCCAAAGAAACAACTTG	TCGAAGATGCCGAGAGAAAA	42.9	45.0	57.7	57.0	318 bp	1.0	62.4