Hotspot

ndhF

SSC · 111169-113394 · 2226 bp

Back to Myristica

Pi 0.00274

MarkerSeek score 46.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 13
pi: 0.00274
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGACAAACAAAGACACATTCA	TGCTCCTTCCGAAAGAGAGA	33.3	50.0	53.9	58.1	2330 bp	1.0	40.4
ndhF_p2	AAGACAAACAAAGACACATTCA	TGCTCCTTCCGAAAGAGAGA	31.8	50.0	54.7	58.1	2331 bp	1.0	40.4
ndhF_p3	AGACAAACAAAGACACATTCAA	TGCTCCTTCCGAAAGAGAGA	31.8	50.0	54.7	58.1	2330 bp	1.0	40.4
ndhF_p4	AAGACAAACAAAGACACATTCAA	TGCTCCTTCCGAAAGAGAGA	30.4	50.0	55.5	58.1	2331 bp	1.0	40.4
ndhF_p5	AGACAAACAAAGACACATTCAAA	TGCTCCTTCCGAAAGAGAGA	30.4	50.0	55.5	58.1	2330 bp	1.0	40.4