Hotspot

ndhA

SSC · 120956-123129 · 2174 bp

Back to Myristica

Pi 0.00069

MarkerSeek score 52.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2174
variable_sites: 3
pi: 0.00069
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CATGAACCCAGTCACCAGAG	GTCCACCGGGTTTCATCAAT	55.0	50.0	57.9	58.2	2398-2402 bp	1.0	78.0
ndhA_p2	TCATGAACCCAGTCACCAGA	GTCCACCGGGTTTCATCAAT	50.0	50.0	58.3	58.2	2399-2403 bp	1.0	76.6
ndhA_p3	CATGAACCCAGTCACCAGAG	TTGCAAATTCTCCCTCAGCT	55.0	45.0	57.9	57.4	2378-2382 bp	1.0	74.6
ndhA_p4	CATGAACCCAGTCACCAGAG	TCCACCGGGTTTCATCAATT	55.0	45.0	57.9	57.4	2397-2401 bp	1.0	74.5
ndhA_p5	TTCATGAACCCAGTCACCAG	GTCCACCGGGTTTCATCAAT	50.0	50.0	57.4	58.2	2400-2404 bp	1.0	74.3