Hotspot

trnN-GUU-trnR-ACG

SSC · 130885-131531 · 647 bp

Back to Myristica

Pi 0.00742

MarkerSeek score 53.50

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 647
variable_sites: 10
pi: 0.00742
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	AACAGATCCAGTGGAGACGA	55.0	50.0	58.0	58.1	798-824 bp	1.0	83.8
trnN-GUU-trnR-ACG_p2	CGGTTGGCTGTTAACTGACT	AACAGATCCAGTGGAGACGA	50.0	50.0	58.1	58.1	768-794 bp	1.0	83.0
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	762-788 bp	1.0	82.9
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	AACAGATCCAGTGGAGACGA	50.0	50.0	57.8	58.1	762-788 bp	1.0	82.8
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	AACAGATCCAGTGGAGACGA	50.0	50.0	58.2	58.1	749-775 bp	1.0	82.8