Hotspot

ndhC-trnV-UAC

LSC · 52384-54282 · 1899 bp

Back to Myristica

Pi 0.0019
MarkerSeek score 59.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1899
variable_sites
8
pi
0.0019
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TAGGGGCTAAAACTCCGGAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 2028-2033 bp 1.0 78.5
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 2026-2031 bp 1.0 77.8
ndhC-trnV-UAC_p3 ATAGGGGCTAAAACTCCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 2029-2034 bp 1.0 77.4
ndhC-trnV-UAC_p4 TAGGGGCTAAAACTCCGGAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.0 57.5 2027-2032 bp 1.0 75.8
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 2025-2030 bp 1.0 75.0