Hotspot

rps16-trnQ-UUG

LSC · 6124-7089 · 966 bp

Back to Myristica

Pi 0.00352
MarkerSeek score 52.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
966
variable_sites
7
pi
0.00352
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1040-1044 bp 1.0 78.8
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1044-1048 bp 1.0 77.7
rps16-trnQ-UUG_p3 CCGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1055-1059 bp 1.0 77.0
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TACTCGGAGGTTCGAATCCT 45.0 50.0 58.1 57.6 1046-1050 bp 1.0 76.0
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA ACTCGGAGGTTCGAATCCTT 45.0 50.0 58.1 58.4 1045-1049 bp 1.0 75.9