Hotspot

ndhD-psaC

SSC · 115727-115893 · 167 bp

Back to Myriopteris

Pi 0.155

MarkerSeek score 80.30

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 167
variable_sites: 24
pi: 0.155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TAGCGTTAGCCGAGGTACAT	GGATCTGAAACTACCCGCAG	50.0	55.0	58.3	58.1	224-236 bp	1.0	89.0
ndhD-psaC_p2	GCGTTAGCCGAGGTACATTA	GGATCTGAAACTACCCGCAG	50.0	55.0	57.5	58.1	222-234 bp	1.0	87.6
ndhD-psaC_p3	TTGGGAAGGAAACGACTAGC	GGATCTGAAACTACCCGCAG	50.0	55.0	57.5	58.1	240-252 bp	1.0	87.5
ndhD-psaC_p4	GACTAGCGTTAGCCGAGGTA	GGATCTGAAACTACCCGCAG	55.0	55.0	58.7	58.1	227-239 bp	1.0	86.0
ndhD-psaC_p5	TAGCGTTAGCCGAGGTACAT	GATCTGAAACTACCCGCAGT	50.0	50.0	58.3	57.3	223-235 bp	1.0	84.1