Hotspot

rpl32-trnP-GGG

SSC · 111812-112417 · 606 bp

Back to Myriopteris

Pi 0.115

MarkerSeek score 81.10

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: SSC
length_bp: 606
variable_sites: 68
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	ATCTTACACAGCTGCTTCGG	CTCTGGCACTGCTTCCTAAG	50.0	55.0	58.0	58.0	1468 bp	0.5	59.7
rpl32-trnP-GGG_p2	ATCTTACACAGCTGCTTCGG	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.0	1466 bp	0.5	59.7
rpl32-trnP-GGG_p3	CCGAATCTTACACAGCTGCT	CTCTGGCACTGCTTCCTAAG	50.0	55.0	58.0	58.0	1472 bp	0.5	59.7
rpl32-trnP-GGG_p4	CCGAATCTTACACAGCTGCT	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.0	1470 bp	0.5	59.7
rpl32-trnP-GGG_p5	GCAAATCCGTAGAGAAGGCA	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.0	1067 bp	0.5	59.6