Hotspot

trnN-GUU-ndhF

SSC · 105969-108547 · 2579 bp

Back to Myriopteris

Pi 0.495

MarkerSeek score 78.40

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 2579
variable_sites: 1078
pi: 0.495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	GTCCAACGCGACTTGATTTG	AATCCTGGTCGTTGAATCGG	50.0	50.0	58.0	58.0	2985 bp	0.5	59.4
trnN-GUU-ndhF_p2	GTCCAACGCGACTTGATTTG	TTTCAAGTTTTGATCGGCGC	50.0	45.0	58.0	58.0	2901 bp	0.5	59.2
trnN-GUU-ndhF_p3	CCTCCACTTGTTGGTGTTGA	TTTCAAGTTTTGATCGGCGC	50.0	45.0	57.9	58.0	2970 bp	0.5	59.2
trnN-GUU-ndhF_p4	TCCACTTGTTGGTGTTGAGG	TTTCAAGTTTTGATCGGCGC	50.0	45.0	57.9	58.0	2968 bp	0.5	59.2
trnN-GUU-ndhF_p5	GTCCAACGCGACTTGATTTG	AATCTTTTTGCAGGGGAGGG	50.0	50.0	58.0	58.1	2845 bp	0.5	59.1